Gene expression and genetic analysis reveal diverse causes of recessive self-compatibility in Brassica napus L.

BMC Genomics

Table 3 Genotypes and phenotypes in segregating populations

Population	Genotype^a			SI/SC phenotype	Expected ratio	χ ²
	S₁₃₀₀	S _BnS-7	S₁₃₀₀S_BnS-7
Year 2012
(S-1300 × 1728) × S-1300	84/0^b		78/0	162/0	1:0	0
(S-1300 × 1728) × 1728		11/28	27/9	38/37	1:1	0.03
(S-1300 × 1728) F₂	33/0	12/26	48/7	93/33	3:1	0.1
(S-1300 × 614) × S-1300	111/0		105/0	216/0	1:0	0
(S-1300 × 614) × 614		9/69	65/15	74/84	1:1	0.64
(S-1300 × 614) F₂	35/0	9/31	71/12	115/43	3:1	0.36
(S-1300 × 1638) × S-1300	56/0		55/0	111/0	1:0	0
(S-1300 × 1638) × 1638		9/49	54/12	63/61	1:1	0.04
(S-1300 × 1638) F₂	37/0	14/27	67/8	118/35	3:1	0.44
(S-1300 × 326) × S-1300	73/0		76/0	149/0	1:0	0
(S-1300 × 326) × 326		93/172	232/22	325/194	1:1	32.56
(S-1300 × 326) F₂	122/0	24/93	196/37	342/130	3:1	1.56
(S-1300 × 230) × S-1300	42/0		38/0	80/0	1:0	0
(S-1300 × 230) × 230		8/48	62/0	70/48	1:1	3.74
(S-1300 × 230) F₂	54/0	7/38	85/0	146/38	3:1	1.63
(S-1300 × 89008) × S-1300	43/0		36/0	79/0	1:0	0
(S-1300 × 89008) × 89008		11/28	31/0	42/28	1:1	2.41
(S-1300 × 89008) F₂	42/0	16/33	87/0	145/33	3:1	4.57
Year 2013
(S-1300 × 614) × S-1300	57/0		64/0	121/0	1:0	0
(S-1300 × 614) × 614		13/33	40/12	53/45	1:1	0.66
(S-1300 × 614) F₂	33/0	11/23	72/15	116/38	3:1	0.03
(S-1300 × 326) × S-1300	61/0		57/0	118/0	1:0	0
(S-1300 × 326) × 326		19/40	53/15	72/55	1:1	2.02
(S-1300 × 326) F₂	33/0	15/24	55/12	103/36	3:1	0.35
(S-1300 × 230) × S-1300	112/0		124/0	236/0	1:0	0
(S-1300 × 230) × 230		16/35	49/0	65/35	1:1	8.41
(S-1300 × 230) F₂	21/0	5/23	54/0	80/23	3:1	0.15
(S-1300 × 89008) × S-1300	30/0	0	25/0	55/0	1:0	0
(S-1300 × 98008) × 89008	0	7/13	21/0	28/13	1:1	4.78
(S-1300 × 89008) F₂	34/0	14/21	71/0	119/21	3:1	6.94

SI: self-incompatible, SCI <2; SC: self-compatible, SCI ≥2.
^aPrimer combinations SRKa-L/SRKa-R and SRK29-1a/SRK29-1b were used to detect BnS-1300 and BnS-7, respectively.
^bNumber of investigated plants with the SI/SC phenotype.

ISSN: 1471-2164