Figure 2
From: Transcriptional response of Saccharomyces cerevisiae to potassium starvation
Antisense transcription of PHO84 . A, scheme showing the position of SAGE tags corresponding to sense and antisense transcripts. B, primers used to detect PHO84 antisense transcripts. C. Detection of antisense transcripts using strand specific cDNA synthesis and PCR. RNA was isolated from cells grown for 60 min in Translucent YNB medium lacking potassium. Arrow indicates the position of the expected PCR fragment.