D . immitis life cycle transcriptome profiles. (A) Relationships between the various D. immitis life cycle stages (AM-Adult Male, AF-Adult Female, mf-microfilariae, L3-3rd stage larva, L4-4th stage larva) as revealed by hierarchical clustering. (B) Clustered transcriptomic data of D. immitis genes across the various life cycle stages. Only genes expressed in at least one stage are shown (n = 12,819). Each gene is represented by a single row. Data from biological replicates were combined prior to clustering. The color scale ranges from black (no expression) to red (very high expression). Black bars indicate the five clusters expressed predominantly in one D. immitis life cycle stage.