Concurrent transcriptional profiling of Dirofilaria immitis and its Wolbachia endosymbiont throughout the nematode life cycle reveals coordinated gene expression

Table 1 Total number of reads sequenced and mapped to the D . immitis genome per D . immitis life cycle stage

Sample	Million reads sequenced (Rep 1)	Million reads mapped (Rep 1)	Million reads sequenced (Rep 2)	Million reads mapped (Rep 2)	Million reads mapped (Reps Combined)	50th Percentile (FPKM)	75th Percentile (FPKM)	95th Percentile (FPKM)	Average coverage (FPKM)/Transcript	D. immitistranscripts detected (%)**
AM	9.3	4.2	2.6	1.0	5.2	3.0	6.7	31.3	83.9	81
AF	14.8	7.2	1.4	0.5	7.7	7.3	14.9	53.4	92.0	93
mf	4.6*	1.6*	43.3	24.7	24.7	8.2	15.0	78.6	53.3	86
L3	20.7	8.7	27.3	12.2	20.9	0.8	2.1	15.3	45.4	81
L4	53.3	33.7	38.3	23.4	57.1	10.4	27.9	129.3	74.3	83

Each life stage replicate, as well as the combined total of mapped reads per life stage are listed. *Due to DNA contamination, this replicate was omitted from further analysis. **Based on number of transcripts expressed (FPKM > 0) per life cycle stage.

ISSN: 1471-2164