Figure 2

Normalized gene expression levels for taste receptor/nutrient sensor genes in circumvallate papillae of pigs. Each bar represents the average gene expression level for 6 biological replicates and the error bars represent the standard error of the mean. The different categories of genes are (A) Taste receptors type 1, (B) Fatty acid receptors, (C) Amino acid and peptone receptors and (D) Bitter taste receptors or Taste receptors Type 2. The normalisation was performed relative to two reference genes RPLP and ACTB and the expression level is provided as a fold change compared to the overall average expression level of Tas1r1 in circumvallate papillae. The letters of the alphabet denote statistical significance, where the same letter refers to no significance (P>0.05) and where a different letter refers to significant differences (P<0.05).