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Table 2 BCL11A and HBS1L-MYB multiplex PCR amplification primers

From: Genotyping of BCL11A and HBS1L-MYB SNPs associated with fetal haemoglobin levels: a SNaPshot minisequencing approach

SNP

Multiplex PCR primers (5′-3′)

mPCR Reaction

Amplicon fragment length (bp)

Concentration (μM)

 

Forward

Reverse

   

BCL11A

     

rs11886868, rs766432

CACACCATGGATGAATCCCAGAα,*

TGGTGCTACCCTGAAAGACGGα,*

B

441

0.1

rs4671393, rs7557939

CCTTGTTTACAGAGGGGCAACCα,*

GCCTTGGGAAGAAAGACAGCATα,*

B

649

0.4

rs6732518

TGGGTGACCCTCTGACTCCT*

GCTTTAACGCACTACACCCCACα,*

A

69

0.1

rs10189857

AGCCATGGTCCACCCACAGAα,*

TTCCAGGTCCCCCAGAAGTAGC*

B

411

0.1

rs6545816

GGTTGATGAGAAAATTACCGCATTAα,*

AACGAGGGTGTTCAAAGTAACCA*

A

103

0.3

rs7599488, rs1427407

CTGGCCGGGAGCATTTCAAα,*

TTTAACCTTCTTAGCACCCACAAA* GCACAGCATGTGACATGATATTCb

B

515

0.6

0.5

rs10184550

CCTGATCTCTGATTGTTGCTTTGA*

TGTACCACCAGAAGTCCTGGAAAα,*

A

120

0.4

rs7606173

ACACCCTGTGATCTTGTGGα,*

GCCAACAGTGATAACCAGC*

A

201

0.8

rs6706648

GAAGCTTCCCCTGTCTGCAα,*

TGAGTGCGTATTTGTAAAGTTCC*

A

333

0.6

HBS1L-MYB

     

rs28384513

CCTTGAGCTACCTACGCCAG*

CTTTCTCAGATTATCAGGAACCAAATTT* GCCCACTGTGTGCTTAATGAAAb

A

66

0.4

0.5

rs7776054, rs9399137

ATATGCAATATTTGTAATTTGTGTTCTGCα,*

TTAACTATATCTGTGCACAGAAATACAGα,*

B

190

0.8

rs9389268, rs9402685

TGCAACCTCCGCTTCCAα,*

TAGCTCACACCTGTAATCATGCα,*

A

221

0.2

rs4895441, rs4895440

GGAAACCAGTTTAGAAAGCGTGG*

TCTCTCTGGATCTCCCTGTCα,*

B

122

0.3

rs6929404

GCAGTGAGATTTCTATTATTAGGCTCα,*

CTGACTAAACTTCTAATCAAAGGCAT*

B

136

0.3

rs9402686

GAGCAGAGAAGTTTAAAGTGTGTG*

TGGACAAAACTGCCCTTTGTCα,*

B

150

0.4

rs1320963

ATGGCTTGGTAAGGACTTAAGAG*

CCTGAGAGTTCTTTTGGGATCTT*

B

60

0.4

TTCAACTTTCCACTGAGTTTTCGb

0.5

rs6904897

ATCCAGGCTTGCCAAATATACCT*

GACTTGCCTGTGCTGGAAATTα,*

A

99

0.3

rs35959442

TGACCCAGAGCGTCCAAGα,*

GTTACATCTGCAGCTGACACC*

A

160

0.8

rs9376090

GATATGGCCAATACCATATGAAGCTAAα,*

AGCTGGGCCTCATTTGTTC*

B

81

0.2

rs9494142

AGGAAGTGTCTTTGGTCTCTCAGα,*

TGAGACTCCATCTCAAAAAACAACA*

A

143

0.4

rs11759553

TTGCCAGGCTGTCTTGCAα,*

GTTCTGCAGGGTCCTTTGG*

B

107

0.2

rs6934903

CCTTGGCCCACATCGCTα,*

TAGAACCTGAAACAGTACTCCACA*

A

130

0.2

  1. *Used as PCR-RFLP primers at final concentration of 0.2 μM as described in the text.
  2. αUsed also as sequencing primers at final concentration of 0.5 μM as described in the text.
  3. bUsed only as sequencing primers as described in the text.