Small RNA profiling reveals regulation of Arabidopsis miR168 and heterochromatic siRNA415 in response to fungal elicitors

Table 1 Elicitor-responsiveness of known Arabidopsis miRNAs as determined by microarray analysis

Name	Sequence	Direction of miRNA expression				Target gene	Biological function
Name	Sequence	5 min	30 min	60 min	120 min	Target gene	Biological function
miR156a	ugacagaagagagugagcac	-	-	-	-1,79	SPL10 TF (At1g27370) ¹	Development
miR156h	ugacagaagaaagagagcac	-4,13	-2,74	1,41	-	SPL2 ( At5g43270) ²	Development
miR164a	uggagaagcagggcacgugca	-	1,16	-	-	CUC1/2 TF (At5g53950/At3g15170) ²	Development. Auxin signaling
miR164c	uggagaagcagggcacgugcg	-	9,5	-	-	NAC080 TF/NAC100 TF (At5g07680/At5g61430) ³	Development. Auxin signaling
miR165a	ucggaccaggcuucauccccc	1,31	-	-	-	PHABULOSA TF/ PHAVOLUTA ^TF (At2g34710/At1g30490) ⁴	Development
miR166a	ucggaccaggcuucauucccc	-1,29	-	-	-	PHABULOSA TF/ PHAVOLUTA ^TF (At2g34710/At1g30490) ⁴	Development
miR168	ucgcuuggugcaggucgggaa	2,88	7,57	2,32	2,1	Argonaute1 (AGO1) (At1g48410) ¹	miRNA functioning. Abiotic stress
miR169d	ugagccaaggaugacuugccg	-	-	-	-2,32	ATHAP2B (At3g05690) ⁵	Development. Auxin signaling
miR170	ugauugagccgugucaauauc	-	-1,76	-	-	SCL TF ⁶	Development
miR415	aacagagcagaaacagaacau	-	5,25	-	-	questioned miRNA
miR418	uaaugugaugaugaacugacc	-	-	-	6,63	questioned miRNA
miR823	uggguggugaucauauaagau	-	10,66	-	-	Chromomethylase 3 (CMT3) (At1g69770) ²	Gene silencing
miR833a-5p	uguuuguuguacucggucuagu	4,55	3,1	2,83	-1,28	F-box containing protein (At1g77650) ²
miR842	ucauggucagauccgucaucc	-	-	-	3,09	Jacalin lectin (At5g28520) ⁷
miR862-5p	uccaauaggucgagcaugugc	-	-1,73	-	-	Unknown

¹[47]²[17]; ³[27]; ⁴[29]; ⁵[2]; ⁶[13]; ⁷[48]. SPL, Squamose promoter binding protein-like; CUC1/2 TF, cup-shaped cotyledon1/2 transcription factor; NAC (NAM, ATAF and CUC) transcription factor; SCL, Scarecrow-like (GRAS TF). -, no change in expression.
miRNAs whose expression varies in at least one time point of elicitor treatment are listed (for details on the entire set of miRNAs represented in the microarray, see Additional file 1: Table S1. The fold change (elicitor-treated vs non-treated plants) for each miRNA is shown. Three biological replicates and three technical replicates for each biological sample were analysed.

ISSN: 1471-2164