Construction of a high-density genetic map based on large-scale markers developed by specific length amplified fragment sequencing (SLAF-seq) and its application to QTL analysis for isoflavone content in Glycine max

Table 4 The characters of 11 QTLs associated with individual and total isoflavone contents across various environments

Name¹	Effect²	Chr³	Interval⁴	IC⁵	LOD⁶	PVE⁷	ADD⁸
qD4	D-E3,E4	4	M634469-M625652	14.67-18.75	6.98	16.00	23.40
qIF7	G-E2,E3; MD-E2,E3; MG-E2,E3; TOT-E2,E3	7	M519691-M491368	33.21-38.13	8.17	14.30	137.00
qMGL7	MGL-E2,E3	7	M523495-M518225	3.62-4.24	4.59	13.30	−23.00
qGL8-2	GL-E2,E4	8	M1060220-M1053451	130.48-132.34	12.40	28.00	8.98
qIF8	MG-E1,E4; TOT-E4	8	M1045556-M1053803	101.44-101.76	5.73	16.90	144.00
qIF13	D-E2,E3,E4; G-E3; MG-E3; MD-E4	13	M153314-M163112	45.77-51.16	4.91	12.50	−44.00
qMGL14	MGL-E1,E3	14	M542764-M545951	6.83-10.60	4.00	8.61	9.96
qMG14	MG-E1,E3	14	M575406-M531913	0.00-2.57	3.50	9.54	83.10
qIF16-1	D-E2,E3; TOT-E2	16	M889900-M897989	20.85-21.19	3.96	8.56	−49.00
qIF16-2	MD-E1,E3,E4;D-E4	16	M895322-M899870	59.53-63.53	5.12	15.10	−79.00
qIF20-2	D-E1,E2,E3,E4; G-E2,E3,E4; MD-E1,E2,E3, E4;MG-E2,E3; TOT-E1,E2,E3	20	M943408-M941848	3.83-8.43	9.11	19.62	117.00

¹The name of QTL, is a composite of the influenced trait: genistin (G), daidzin (D), glycitin (GL), malonyldaidzin (MD), malonylgenistin (MG), malonylglycitin (MGL) and total isoflavones (TOT) followed by the chromosome number. For QTLs underlying the contents of multiple isoflavone components, the name is a composite of isoflavones (IF) followed by the chromosome number.
²The Effect of QTL is composite of the particular isoflavone component followed by the specific environments. It represents the particular isoflavone components [i. e., genistin (G), daidzin (D), glycitin (GL), malonyldaidzin (MD), malonylgenistin (MG), malonylglycitin (MGL)] and total isoflavones (TOT) that are controlled by this QTL in specific environments [i. e., E1 (2009 at Changping), E2 (2009 at Shunyi), E3 (2010 at Shunyi), and E4 (2011 at Shunyi)].
³Chr indicates chromosome.
⁴Interval indicates confidence interval between two SLAF markers.
⁵IC indicates the interval of confidence in centimorgan.
⁶LOD indicates the logarithm of odds score.
⁷PVE indicates the phenotypic variance explained by individual QTL.
⁸ADD indicates the additive effect value.
The LOD scores, PVE values, and additive effect values are shown as mean values for QTLs with multiple effects.

ISSN: 1471-2164