Construction of a high-density genetic map based on large-scale markers developed by specific length amplified fragment sequencing (SLAF-seq) and its application to QTL analysis for isoflavone content in Glycine max

Table 5 Additive effect of qIF20-2 for individual and total isoflavone contents across various environments

Name¹	Effect²	Interval³	IC⁴	LOD⁵	PVE⁶	ADD⁷
qIF20-2	D-E1	M934616-M970087	5.92-6.63	4.33	15.16	22.27
	D-E2	M947267-M941848	7.63-8.43	6.14	15.60	24.72
	D-E3	M934616-M970087	5.92-6.63	9.47	21.13	34.72
	D-E4	M934616-M970087	5.92-6.63	6.51	15.38	14.25
	G-E2	M947267-M941848	7.63-8.43	9.63	21.96	31.59
	G-E3	M934616-M970087	5.92-6.63	11.08	24.19	63.09
	G-E4	M947267-M941848	7.63-8.43	5.00	12.28	24.91
	MD-E1	M943408-M968883	3.83-4.64	4.93	17.89	104.92
	MD-E2	M934616-M970087	5.92-6.63	9.36	23.35	152.44
	MD-E3	M934616-M970087	5.92-6.63	9.08	20.13	125.71
	MD-E4	M934616-M970087	5.92-6.63	4.52	12.55	75.15
	MG-E2	M934616-M970087	5.92-6.63	18.00	27.89	129.48
	MG-E3	M968883-M934616	4.64-5.92	9.64	23.41	156.97
	TOT-E1	M934616-M970087	5.92-6.63	2.86	8.67	151.54
	TOT-E2	M934616-M970087	5.92-6.63	16.25	19.04	280.27
	TOT-E3	M968883-M934616	4.64-5.92	18.89	35.29	472.78

¹The name of QTL, is a composite of the influenced trait: genistin (G), daidzin (D), glycitin (GL), malonyldaidzin (MD), malonylgenistin (MG), malonylgenistin (MGL) and total isoflavones (TOT) followed by the chromosome number. For QTLs underlying the contents of multiple isoflavone components, the name is a composite of isoflavones (IF) followed by the chromosome number.
²The Effect of QTL is composite of the particular isoflavone component followed by the specific environments. It represents the particular isoflavone components [i. e., genistin (G), daidzin (D), glycitin (GL), malonyldaidzin (MD), malonylgenistin (MG), malonylglycitin (MGL)] and total isoflavones (TOT) that are controlled by this QTL in specific environments [i. e., E1 (2009 Changping), E2 (2009 Shunyi), E3 (2010 Shunyi), and E4 (2011 Shunyi)].
³Interval indicates confidence intervals between two SLAF markers.
⁴IC indicates the interval of confidence in centimorgan.
⁵LOD indicates the logarithm of odds score.
⁶PVE indicates the phenotypic variance explained by individual QTL.
⁷ADD indicates the additive effect value.

ISSN: 1471-2164