Figure 2
From: Extensive duplication of the Wolbachia DNA in chromosome four of Drosophila ananassae
Confirmation of coverage metrics using a qPCR based assay. The copy number was confirmed and found to be accurate up to 8 copies per haploid genome using a qPCR assay measuring the ratio of the LGT abundance to that of six single copy Drosophila genes as shown for the 3 kbp mate pair library for the cured Hawaii (Panel A), India (Panel B), and Indonesia (Panel C). A significant positive correlation (p < 0.001, linear regression) is found for the cured Hawaii and the India line The results in Panels A and C represent the accumulation of multiple qPCR experiments while those in Panel B result from a single experiment.