Figure 1

Signatures of gene body methylation are bimodally distributed in the coral. (A) Distribution of genes based on normalized CpG content. The green curve indicates the low-CpG component (predicted to be strongly methylated). The red curve indicates the high-CpG component (predicted to be weakly methylated). The black dotted line separates the two components at the point of intersection between the curves. (B) Distribution of genes based normalized GpC content. In contrast to CpG dinucleotides, GpCs are not targeted for methylation so a normal distribution is expected. (C) Negative linear relationship between CpG_O/E and TpG_O/E. This is consistent with the prediction that DNA methylation causes depletion of CpG content largely though substitution of methylated cytosines for thymine.