Figure 4

Average ribosome depth of genes mapped using 3′ end reads. Genes longer than 500 bp were selected, and ribosome depths, from -20 to +480, from the 5′ end of the gene, were normalized to an average value of one, then the depths in all genes were averaged. The ribosome depth calculated using 3′ end of sequence reads were used and the samples treated with Cm (A) or Tet (B) to inhibit translation was shown.