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Figure 1 | BMC Genomics

Figure 1

From: Evaluation and validation of a robust single cell RNA-amplification protocol through transcriptional profiling of enriched lung cancer initiating cells

Figure 1

Transcriptional profiling of RNA-Amplified MCF7 RNA using three different protocols. Replicate samples of 25-50 pg MCF7 RNA were RNA-Amplified using three commercial kits and 5 μg of the resulting cDNA was analysed on Affymetrix arrays. (A) Miltenyi SuperAmp™ replicates showed 865 genes present across both samples with a correlation of 0.800, (B) NuGEN Ovation One-Direct™ identified 1554 with a correlation of 0.723 and (C) EpiStem RNA-Amp™ identified 2667 present with a correlation of 0.866. (D) Venn diagram showing overlap of genes present in both replicates of Miltenyi, NuGEN and EpiStem samples (all analysis based on p ≤ 0.05).

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BMC Genomics

ISSN: 1471-2164

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