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Figure 3 | BMC Genomics

Figure 3

From: Evaluation and validation of a robust single cell RNA-amplification protocol through transcriptional profiling of enriched lung cancer initiating cells

Figure 3

Comparison of differential expression between amplified and unamplified samples. (A) PCA analysis of DE genes (LIMMA FC > 2, FDR <0.01) identified from Affymetrix array analysis of 10 μg MCF7 and MCF10A RNA samples aligned with the corresponding Affymetrix array data for the single cell and 1 ng amplified MCF7 and MCF10A samples showing clear clustering according to cell type. (B) Heat map of hierarchical clustering of the top 200 differentially expressed genes identified from Affymetrix array analysis of 10 μg MCF7 and MCF10A RNA samples aligned with the corresponding Affymetrix array data for the single cell and 1 ng amplified MCF7 and MCF10A samples. Heat map colour scheme as described in Figure 2B.

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BMC Genomics

ISSN: 1471-2164

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