Figure 6

Transient expression assays by Agrobacterium -mediated vacuum infiltration. (a) Staining for GUS activities in leaves of NL895 infiltrated with five Agrobacterium stains carrying pCambia1305.1. (b) Expression of 12 MbIGYPs in NL895 leaves was detected by Western blot using an anti-GFP antibody. From left to right: protein ladder, MbIGYP59, MbIGYP6, MbIGYP13, MbIGYP50, MbIGYP52, MbIGYP10, MbIGYP43, MbIGYP33, MbIGYP71, MbIGYP79, MbIGYP87 and MbIGYP94. (c) Cell death symptoms on the resistant poplar clone NL895 at 5 dai with AGL1 harboring MbIGYP13. (d) NL895 leaves infiltrated with AGL1 containing the empty vector at 5 dai (negative control). (e) Necrosis phenotype of non-host P. tomentosa leaves at 5 dai after infiltration with AGL1 carrying MbIGYP13. (f) P. tomentosa leaves infiltrated with AGL1 containing the empty vector for 5 days (negative control). Photographs (c)-(f) show 5-6 leaves collected from a single seedling. Experiments were repeated twice with similar results.