Table 1 Differentially expressed muscle proteins in total extract between adult (56 yr) and old (78 yr) post-menauposal women
From: Proteomics of muscle chronological ageing in post-menopausal women
2DGE pH | Spot n° | Accession | Symbol | Protein name | Fold change | Age effect (pvalue) | Mascot score | Sequence coverage (%) | Unique peptides | Main biological function |
|---|---|---|---|---|---|---|---|---|---|---|
5-8 | 1882 | P05976 | MYL1 | Myosin light chain 1/3 | +1.4 | 0.020 | 693 | 41 | 5 | Myofilaments and cytoskeleton |
5-8 | 2032 | P10916 | MYL2 | Myosin regulatory light chain 2 | −1.7 | 0.012 | 4807 | 86 | 15 | Myofilaments and cytoskeleton |
5-8 | 1123 | P45378 | TNNT3 | Troponin T, fast skeletal muscle | +1.9 | 0.033 | 288 | 24 | 4 | Myofilaments and cytoskeleton |
5-8 | 1333 | P68133 | ACTA1 | Actin, alpha skeletal muscle (N-term fragment) | +1.7 | 0.042 | 999 | 23 | 4 | Myofilaments and cytoskeleton |
5-8 | 1415 | P68133 | ACTA1 | Actin, alpha skeletal muscle (N-term fragment) | +1.3 | 0.034 | 847 | 29 | 4 | Myofilaments and cytoskeleton |
5-8 | 1417 | P68133 | ACTA1 | Actin, alpha skeletal muscle (C-term fragment) | +1.3 | 0.041 | 350 | 19 | 4 | Myofilaments and cytoskeleton |
5-8 | 570 | P12882 | MYH1 | Myosin-1 (C-term fragment) | +2.3 | 0.011 | 712 | 9 | 11 | Myofilaments and cytoskeleton |
3-5.6 | 665 | P31415 | CASQ1 | Calsequestrin-1 | +1.4 | 0.045 | 1146 | 22 | 9 | Signal transduction |
5-8 | 858 | Q86TD4 | SRL | Sarcalumenin (mature C-term) | −1.6 | 0.008 | 1606 | 44 | 17 | Signal transduction |
5-8 | 3260 | Q9NP98 | MYOZ1 | Myozenin-1 | +1.7 | 0.008 | 435 | 22 | 4 | Signal transduction |
3-5.6 | 758 | P20073 | ANXA7 | Annexin A7 | +1.8 | 0.029 | 954 | 15 | 6 | Signal transduction, autophagy |
5-8 | 300 | P06732 | CKM | Creatine kinase M-type (dimer) | −1.7 | 0.031 | 445 | 25 | 7 | Energy metabolism |
5-8 | 332 | P06732 | CKM | Creatine kinase M-type (dimer) | −1.7 | 0.048 | 737 | 39 | 11 | Energy metabolism |
5-8 | 391 | P11217 | PYGM | Glycogen phosphorylase, muscle form | −2.6 | 0.031 | 1516 | 39 | 23 | Energy metabolism, glycogenolysis |
5.3-6.5 | 1874 | P21695 | GPD1 | Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic | −1.4 | 0.040 | 3383 | 53 | 17 | Energy metabolism, shuttle |
5-8 | 3459 | Q8N335 | GPD1L | Glycerol-3-phosphate dehydrogenase 1-like protein | −1.4 | 0.022 | 1884 | 55 | 14 | Energy metabolism, shuttle, hypoxia |
5-8 | 3511 | P60174 | TPI1 | Triosephosphate isomerase | +1.3 | 0.050 | 798 | 56 | 9 | Energy metabolism, glycolysis, detoxification |
5-8 | 1196 | P11177 | PDHB | Pyruvate dehydrogenase E1 component subunit beta | −1.3 | 0.043 | 2267 | 59 | 14 | Energy metabolism, Krebs cycle |
5-8 | 769 | P09622 | DLD | Dihydrolipoyl dehydrogenase, mitochondrial | −1.4 | 0.010 | 466 | 29 | 8 | Energy metabolism, Krebs cycle |
5-8 | 577 | Q99798 | ACO2 | Aconitate hydratase, mitochondrial | −1.6 | 0.043 | 365 | 19 | 9 | Energy metabolism, Krebs cycle |
5-8 | 984 | O75306 | NDUFS2 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial | −1.6 | 0.005 | 1777 | 49 | 15 | Energy metabolism, oxidative phosphorylation |
5-8 | 3417 | P47985 | UQCRFS1 | Cytochrome b-c1 complex subunit Rieske, mitochondrial | −1.3 | 0.031 | 1271 | 23 | 5 | Energy metabolism, oxidative phosphorylation |
3-5.6 | 2713 | P20674 | COX5A | Cytochrome c oxidase subunit 5A, mitochondrial | −1.5 | 0.015 | 779 | 48 | 6 | Energy metabolism, oxidative phosphorylation |
5-8 | 909 | P06576 | ATP5B | ATP synthase subunit beta | −1.4 | 0.023 | 2725 | 62 | 20 | Energy metabolism, oxidative phosphorylation |
5-8 | 2371 | P05413 | FABP3 | Fatty acid-binding protein, heart | −1.4 | 0.004 | 2281 | 77 | 11 | Energy metabolism, lipid transport |
5-8 | 3328 | P15121 | AKR1B1 | Aldose reductase | −1.5 | 0.016 | 323 | 31 | 5 | Detoxification, cytoprotection |
3-5.6 | 1414 | P04792 | HSPB1 | Heat shock protein beta-1 | +1.7 | 0.019 | 714 | 36 | 5 | Detoxification, cytoprotection |
5.3-6.5 | 2375 | P04792 | HSPB1 | Heat shock protein beta-1 | +1.3 | 0.026 | 484 | 41 | 6 | Detoxification, cytoprotection |
5-8 | 678 | P08107 | HSPA1A | Heat shock 70 kDa protein 1A/1B | +1.1 | 0.014 | 3630 | 52 | 14 | Detoxification, cytoprotection |
5-8 | 3332 | P38646 | HSPA9 | Stress-70 protein, mitochondrial | +1.4 | 0.008 | 5401 | 45 | 22 | Detoxification, cytoprotection |
5-8 | 3289 | P00918 | CA2 | Carbonic anhydrase 2 | +1.3 | 0.047 | 1605 | 54 | 11 | Detoxification, cytoprotection |
5-8 | 877 | Q13228 | SELENBP1 | Selenium-binding protein 1 | −1.5 | 0.028 | 1677 | 56 | 19 | Detoxification, cytoprotection |
5.3-6.5 | 2410 | P28070 | PSMB4 | Proteasome subunit beta type-4 | −1.4 | 0.049 | 1692 | 33 | 5 | Proteolysis |
3-5.6 | 3387 | P54725 | RAD23A | UV excision repair protein RAD23 homolog A | −1.3 | 0.041 | 309 | 28 | 6 | Proteolysis |
5-8 | 3270 | P49411 | TUFM | Elongation factor Tu, mitochondrial | −1.4 | 0.001 | 1548 | 38 | 12 | Protein synthesis, proteolysis |
5-8 | 919 | P02679 | FGG | Fibrinogen gamma chain | −1.3 | 0.026 | 1023 | 59 | 16 | Serum |
5-8 | 1429 | P30042 | C21orf33 | ES1 protein homolog, mitochondrial | −1.6 | 0.001 | 364 | 51 | 6 | Miscellaneous |
5-8 | 1430 | P30042 | C21orf33 | ES1 protein homolog, mitochondrial | −1.7 | 0.002 | 118 | 18 | 3 | Miscellaneous |
5-8 | 1177 | Q9H0P0 | NT5C3 | Cytosolic 5'-nucleotidase 3 | −1.6 | 0.015 | 589 | 35 | 10 | Miscellaneous |
3-5.6 | 1429 | Q9Y235 | APOBEC2 | Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 2 | −1.2 | 0.018 | 1904 | 74 | 11 | Miscellaneous |