Figure 1From: NextGen sequencing reveals short double crossovers contribute disproportionately to genetic diversity in Toxoplasma gondiiDevelopment of the genetic cross. A) Schematic representation of the genetic cross between type 10 VAND and type 2 ME49 strains. Recombinant progeny were isolated either using dual drug selection or genotyping using eight RFLP makers located on separate chromosomes. B) Confirmation of recombinant progenies by PCR-RFLP markers. Markers are listed to the left, and restriction enzymes used in the digest to the right. V, VAND SNFR; M, ME49 FUDRR, clone names are indicated above the gel. –Ve, negative control.Back to article page