Analysis of double crossovers. A) Normalized histogram of double crossovers sizes. Double crossovers were defined as two consecutive crossovers that occur within 5,000 bp. The boundaries of the double crossovers were supported by at least six SNPs and the two boundaries were separated by at least five SNPs defining the crossover. The region sizes were counted in steps of 250 bp and normalized by highest count. B) Location of single and double crossovers depicted by Circos plot of the 14 chromosomes. The outside track plots the genes across the 14 chromosomes; top strand (blue) - bottom strand (red). The inside track plots the positions of the double crossovers that occurred in single progeny (red) vs. multiple progeny (blue). The second most inner track plots the positions of the conventional single crossovers (green). The second most outer track plots a histogram (black) of SNPs between VAND and ME49 per 10 kb rolling window, y-axis 0–500. C) Classification of the coding capacity of genes containing double crossovers. A total of 60 genes were identified that span a double crossover boundary. KEGG/Gene Ontology annotations obtained from ToxoDB.org were grouped based on functional categories. Chart represents percentage of the genes within each category.