Experimental workflow to identify kinase targets using high-content imaging. (A) Cells were fluorescently labeled for DNA (DAPI staining, blue channel) and for phosphorylated histone H3 (green channel) as indicated above the photographs. Using CellProfiler software, snapshots were split into blue and green channels. For each image a local correction was applied and objects (nucleus and phosphohistone H3-positive cells) were counted. (B) Normalization and Mitotic Index quantification were performed using CellHTS2 free software. The diagram depicts box-plots of the whole experiment.