Expected methylation (%) | Average methylation ± standard deviation (%) | ||||
---|---|---|---|---|---|
qPCR | dPCR | ||||
P14_M | P14_M | P14_M | P14_M2 | P14_M + P14_M2 | |
Singleplex | Singleplex | Duplex | Duplex | Duplex | |
100 | 100* | 100 | 100 | 100 | 100 |
90 | 78 ± 4¥ | 77 ± 27 | 99 ± 19* | 86 ± 20 | 92 ± 19 |
75 | 66 ± 18* | 81 ± 13 | 86 ± 20 | 77 ± 17 | 81 ± 19 |
50 | 49 ± 10¥,+ | 52 ± 16 | 58 ± 10*,¥ | 57 ± 28* | 56 ± 18* |
25 | 28 ± 4‡ | 21 ± 7 | 31 ± 5 | 28 ± 2 | 29 ± 4 |
10 | 13 ± 3+ | 17 ± 4 | 18 ± 6¥ | 9 ± 15* | 13 ± 10* |
0 | 0‡ | 0 | 0 | 0 | 0 |