Figure 2

Fluorescent in situ hybridization (FISH) for selected differentially expressed genes on fertile colonies. FISH for selected genes with homologs in humans and other organisms was performed on whole mounts of adult fertile colonies. Nuclei were stained with DAPI (blue) and riboprobes are shown in green. Based on their expression patterns in developing gonads, these genes fall into four distinct categories: 1) (A-E) localized to testes or developing testes (otoa, tspan8, tsk1, vtg1); 2) (F-I) localized to maturing eggs (vtg1, ldlr, zp1, tll1); 3) (J-N) oocytes at stage 2 (hsd17β8, selp, tsk2, tspn8); and 4) (O-R) other tissues (zp1, selp, and ldlr). otoa = otoancorin, tspan8 = tetraspanin-8, tsk1 = testis-specific serine/threonine-protein kinase1, tsk2 = testis-specific serine/threonine-protein kinase2, vtg1 = vitellogenin, ldlr = Low-density lipoprotein receptor 1, zp1 = zona-pellucida sperm binding protein 1, tll1 = Tolloid-like protein1, hsd17β8 = Estradiol 17β dehydrogenase-8, selp = P-selectin. Scale bar 50 μm.