Figure 3

Fluorescent in situ hybridization (FISH) for selected differentially expressed genes on infertile colonies. FISH for selected genes was performed on whole mounts of adult infertile colonies (A-J). Nuclei were stained with DAPI (blue) and riboprobes are shown in green. Expression of genes detected in fertile colonies is absent in juveniles except for p-selectin (localizing to both oral and excurrent siphons) as indicated by arrows (B) and Zona-pellucida sperm binding protein1 (localizing to the endostyle) as indicated by the arrowhead (H). otoa = otoancorin, tspan8 = tetraspanin-8, tsk1 = testis-specific serine/threonine-protein kinase1, tsk2 = testis-specific serine/threonine-protein kinase2, vtg1 = vitellogenin, ldlr = Low-density lipoprotein receptor 1, zp1 = zona-pellucida sperm binding protein 1, tll1 = Tolloid-like protein1, hsd17b8 = Estradiol 17β dehydrogenase-8, selp = P-selectin. Scale bar 200 μm.