Active chromatin marks overlap with repressed RTSSs. The figure shows data for HeLa-S3for all RTSS and isolated RTSS. Data for isolated RTSSs defined as RTSSs separated by at least 2kbp from its nearest neighboring RTSS. The p-values also confirmed highly significant overlaps of active marks with repressed RTSSs, especially for the marks DNase HS, H2A.Z, H3K4me1, H3K4me2, H3K4me3, H3K27ac, H3K9ac and H4K20me1, but less significant overlap with the transcriptional marks H3K36me3 and H3K79me2 [Additional file 1: Table S18]. Plots for the other three cell lines are in [Additional file 1: Figure S1].