Figure 2

Complex aneuploidy triggers similar pathway changes as trisomy or tetrasomy. A. Schematic depiction of the construction of aneuploid cell lines with complex karyotype changes. B. Chromosome numbers in clonal cell lines originating from an unstable tetraploid intermediate. The numbers above the plot indicate the median chromosome number. C. 2-dimensional pathway analysis comparison of the complex aneuploid cell line HPT1* and HCT116 5/4. D. 2-dimensional comparison of complex aneuploid cell line HPT2* and DLD1 13/3. E. Distance matrix of the Spearman rank correlation of all analyzed aneuploid cell lines. Large distances present a low correlation and vice versa. The Spearman distance metric is defined as distanceSpearman = (1- correlationSpearman)/2 in the distanceMatrix function as part of the R Class Discovery package. The function calculates the distance of the correlation between column vectors, each of which represents different microarray experiments. Asterisks indicate the cell lines with H2B-GFP.