Skip to main content
Figure 8 | BMC Genomics

Figure 8

From: Transcriptome sequencing of Atlantic salmon (Salmo salar L.) notochord prior to development of the vertebrae provides clues to regulation of positional fate, chordoblast lineage and mineralisation

Figure 8

Notochord morphology and in situ hybridization of col11a2 . (A, B) show the morphology before, during and after mineralisation start in the notochord sheath. (A) The non-segmented notochord at 500 d°. Arrow: notochord sheath; arrowhead: chordoblasts; ca: cartilaginous arcualia. Scale bar: 100 um. (B) Notochord at start of mineralisation in the sheath (arrow), and slightly segmented chordoblasts (arrowhead). Scale bar: 100 um. (C) Tissue-specific, segmental gene expression of col11a2 antisense in chordoblasts (arrowhead) in the notochord at 710 d°. No staining in the notochordal sheath (arrow). Scale bar: 20 μm. (D) No background signal was found using the sense probe of col11a2 (710 d°). Arrowhead: chordoblasts, arrow: notochord sheath. Scale bar: 20 μm. (E) Notochord and surroundings, 1000 d°. Red arrow indicates the mineralised chordacentrum, the earliest vertebral element. Ca: arcualial cartilage. (F) Differentiated chordacentrum (red arrow), 1300 d°. Notice the thickened notochordal sheath between segments (il: intervertebral ligament), and the thin remaining part of the sheath. Ca: arcualial cartilage. Scale bar: 100 μm.

Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us