Table 3 Characteristics of EST-SSRs and efficiency of marker development in cabbage
Motif | No. of EST-SSRs (%) | No. of designed primer pairs | No. of primer pairs amplifying product (%)a | No. of polymorphic primers (%)b |
|---|---|---|---|---|
Dinucleotide | 750 (31.19) | 223 | 183 (82.06) | 26 (14.21) |
  AC/CA | 73 (3.04) | 29 | 26 (89.66) | 4 (15.38) |
  AG/GA | 543 (22.58) | 162 | 130 (80.25) | 18 (13.85) |
  AT/TA | 134 (5.57) | 32 | 27 (84.38) | 4 (14.81) |
Trinucleotide | 1,591 (66.15) | 645 | 589 (91.32) | 78 (13.24) |
  AAC/ACA/CAA | 151 (6.28) | 64 | 57 (89.06) | 9 (15.79) |
  AAG/AGA/GAA | 484 (20.12) | 195 | 171 (87.69) | 20 (11.70) |
  AAT/ATA/TAA | 26 (1.08) | 11 | 10 (90.91) | 2 (20) |
  ACC/CAC/CCA | 143 (5.95) | 60 | 57 (95) | 10 (17.54) |
  ACG/CGA/GAC | 25 (1.04) | 7 | 7 (100) | - |
  ACT/CTA/TAC | 39 (1.62) | 19 | 16 (84.21) | 2 (12.5) |
  AGC/GCA/CAG | 135 (5.61) | 52 | 50 (96.15) | 5 (10) |
  AGG/GGA/GAG | 228 (9.48) | 103 | 98 (95.15) | 15 (15.31) |
  ATC/TCA/CAT | 299 (12.43) | 111 | 101 (90.99) | 13 (12.87) |
  CCG/CGC/GCC | 61 (2.54) | 23 | 22 (95.65) | 2 (9.1) |
Tetranucleotide | 24 (1) | 15 | 13 (86.67) | 2 (15.38) |
Pentanucleotide | 12 (0.5) | 4 | 3 (75) | 1 (33.33) |
Hexanucleotide | 28 (1.16) | 11 | 10 (90.91) | 2 (20) |
Compound formation | 92 | 39 | 31 (79.49) | 7 (22.58) |
Total | 2405 (100) | 937 | 829 (88.47) | 116 (13.99) |