Figure 3
The erg1 mRNA increases its association with polysomes during ER stress. Mycelial extracts from control (untreated) and TM-treated cultures were fractionated into 7 pools. The RNA in each pool was then separated by RNA gel electrophoresis and the level of erg1 mRNA in each fraction was determined by hybridization to an erg1 probe. Band intensities were quantified by phosphorimager analysis and shown on the top graph. A representative OD254 profile is superimposed on the graph for reference. The findings demonstrate increased erg1 mRNA levels in the polysome fraction during ER stress.