Figure 8

Northern blot analysis of yvc1 expression. RNA was isolated from the indicated strains in the presence or absence of ER stress (1 mM DTT for 1 h, 10 μg/ml TM for 1 h) or osmotic stress (0.8 M NaCl for 1 h). The blot was hybridized with the full-length A. fumigatus yvc1 gene, then stripped and re-probed with the UPR marker bipA. Accuracy of RNA loading is shown by SYBR-green staining of rRNA.