Regions of variability among the B. breve genomes. a) BLAST-based genome atlas showing the presence of each ORF from B. breve UCC2003 and the other B. breve complete representatives. From outer to inner circle: B. breve UCC2003, B. breve JCM 7017, B. breve 689b, B. breve NCFB 2258, B. breve S27, B. breve JCM 7019, B. breve 12L, B. breve ACS-071-V-Sch8b. b) Alignment showing in red the ORFs with significant G+C mol% in B. breve (B. breve UCC2003, B. breve 689b, B. breve 12L, B. breve NCFB 2258, B. breve S27, B. breve JCM 7017, B. breve JCM 7019, B. breve ACS-071-V-Sch8b); highlighted in grey are regions of variability (REG1-8) that were identified based on gene presence/absence and G+C mol% deviation in each strain. c) Hierarchical clustering heatmap representing the variability of B. breve in terms of presence/absence of genes for eight complete genomes of B. breve with associated percentage of core and variable gene families represented as pie chart.