Bayesian phylogenetic tree representing the molecular evolutionary history of the Shiva superfamily toxins. The tree shows the split between the three main toxin classes (ω, κ, and ω/κ). ω-Actinopoditoxin-Mb1a from the Eastern mouse spider Missulena bradleyi was used as the outgroup. Toxins belonging to each species are highlighted in the following colours: H. versuta, red; H. modesta, black; H. venenata, green; H. infensa, magenta; A. robustus, pale blue; H. formidabilis, dark blue; ω-actinopoditoxin-Mb1a from M. bradleyi, orange. *denotes a species not sequenced as part of this study; the sequence was downloaded from UniProt under accession number P83588.