Figure 2

Evaluation of gene expression levels on the four chromosomes of Fusarium graminearum . (A) The expression levels of genes on each chromosome were quantified by the number of reads per kilobase per million reads mapped. Genes on chromosome 2 showed relative low expression levels. Different letters represent statistically significant differences (P < 0.01, one-way ANOVA). (B) All genes of F. graminearum were grouped into five classes according to their RPKM values (0, 0–1, 1–10, 10–100, >100), and the proportion of each class was calculated for each chromosome. (C) Box plot analysis of gene expression on each chromosome. The expression of each gene was quantified by its RPKM value. Log2-transformed RPKM values were used to draw a box plot graph for each chromosome.