Comparative growth analysis of Ssa1-4 in response to H
. Overnight culture was diluted in fresh YPD medium to an OD600nm = 0.1 and then the cells were grown to an exponential phase to a density of 3 × 106 cells/ml. Cells were then re-suspended in fresh medium to a density of 5 × 106 cells/ml and transferred to a microtitre plate. Representative spots shown in the figure are a neat concentration from a 1 in 5 serial dilution series. The plates were incubated for 3 days at 30°C.