Immunoblot demonstrating the expression of IGLL5 in carotid plaques obtained at endarterectomy. Lanes 1–3: lysates from an area exhibiting diffuse intimal thickening (DIT). Lanes 4–6: matched lysates of fibroatheromatous plaque from the same samples. Panel A: anti-human IGLL5 antibody staining. Panel B: Beta-actin band used for densitometric quantification of IGLL5. Panel C: Increased trends of IGLL5 expression in fibroatheromatous plaques. See also Additional file 1: Figure S3 for siRNA knockdown experiments of to establish specificity of this antibody for IGLL5.