Table 4 Primers used in real-time quantitative PCR of Lilium lancifolium (RT-qPCR)
From: Transcriptome profiling of the cold response and signaling pathways in Lilium lancifolium
Unigene Id | Gene name | Annotation | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) | Correlation between RNA-Seq and qRT-PCR(r2) | GenBank accession numbers |
|---|---|---|---|---|---|---|
Contig15860 | LlAP2 | Putative AP2/EREBP transcription factor | CCGCCCTCTTCAATCTCATC | TATCTGGCTCGGCTCCTAC | 0.98 | KJ489026 |
Contig18777 | LlLEA | LEA-like protein | AAGATGTTCCTCCTCGTGTTG | GATGTTGGTCCTCGCCTTC | 0.99 | KJ489024 |
Contig11020 | LlCIS | Peptidyl-prolyl cis-trans isomerase | TTGTTCCTTCCACCGCATTA | AAAGCCTTCATCCTCAAACTTAGAC | 0.95 | KJ467624 |
Contig9406 | LlDREB1 | DRE-binding protein | AAATCCGCCTCCCCAAGAA | AGTTGAGCCGAGCGAAGT | 0.98 | KJ467618 |
Contig1500 | LlHOT | Heat shock protein | ATGATTGGGAGGAGCACTTG | GAAGACACGGCGGACATAA | 0.97 | KJ467620 |
Contig13202 | LlCOR12 | Cold-regulated LTCOR12 | CGGACACAACTTGACTCTTACC | CTTGCTATGCCTCGCTGAC | 0.94 | KJ489025 |
Contig1641 | LlMYBR | MYBR domain class transcription factor | TTCCTCAGTCCACGCTATCC | GCCGTTGCCTAACTACTTGTC | 0.98 | KJ467623 |
Contig25399 | LlNAC1 | NAC domain protein | GGTTTAGAGGGAGGTTGGAGAAG | CGACGACACTGGCTCATCA | 0.94 | KJ467622 |
Contig22048 | LlCDPK | Calcium-dependent protein kinase | GTCGTGCTCCAATTACCAGAAG | CAAGAGGAACAACATCACCAGAC | 0.94 | KJ467621 |
Contig5309 | LlGPAT | GPAT | TGCAAAGTGGAAATCCTAATGCGAG | AGGATCTGCTTCCGTCTGATGGTTT | 0.97 | KJ467617 |
| Â | LlTIP1 | Reference gene | CGAAGCCAGAAACGGAGAAGAAT | GGGTAGGGTGGATTGGGAAGA | Â | Â |