Figure 1

Phylogenetic relationship of canola CPK proteins with CPKs from other species. Protein sequences were aligned using ClustalX (v1.83) and a maximum parsimony (MP) bootstrap consensus tree was drawn using MEGA5.1. The CPKs can be clustered into four major groups (I-IV). The percentage of replicate trees is shown on the branches and it is calculated in the bootstrap test (1000 replicates) for the associated taxa being clustered together. The 23 BnaCPKs reported in this study are highlighted in green. The tree was rooted with a CDPK identified from the smallest free-living organism, Ostreococcus tauri. At, Arabidopsis thaliana; Bna, Brassica napus; Cr, Chlamydomonas reinhardtii; Gh, Gossypium hirsutum; Le, Lycopersicon esculentum; Mt, Medicago truncatula; Nt, Nicotiana tabacum; Os, Oryza sativa; Pp, Physcomitrella patens; Sb, Sorghum bicolor; Sm, Selaginella moellendorffii; St, Solanum tuberosum; Ta, Triticum aestivum; Vf, Vicia faba; Zm, Zea mays, Ol, Ostreococcus lucimarinus; Ot, Ostreococcus tauri.