Bimolecular fluorescence complementation (BiFC) analysis of interactions between BnaCPKs and Clade A BnaPP2Cs or BnabZIP TFs co-expressed in N. benthamiana leaf cells. The coding regions of BnaCPKs and BnaPP2Cs or BnabZIPs were fused to the N- and C-terminal halves of YFP, respectively. The plasmid combinations are indicated at the left side. (A) YFPN-CPK7 coexpressed with HAB1-YFPc; (B) YFPN-CPK7 with HAB2-YFPc; (C) YFPN-CPK7 with YFPc; (D) YFPN-CPK28 with HAB2-YFPc; (E) YFPN-CPK28 with YFPc; (F) YFPN-CPK4 with ABF3-YFPc; (G) YFPN-CPK4 with ABF4-YFPc; (H) YFPN-CPK4 with ABI5-YFPc; (I) YFPN-CPK4 with AREB3-YFPc; (J) YFPN-CPK4 with YFPc. The fluorescence of YFP was observed by confocal laser microscopy. The left panel is YFP fluorescence, the middle bright field and the right represents an overlay of the two images. Bar = 50 μm.