Culture conditions for Cochliobolus lunatus strain btl5 (GenBank®accession JX907828) following two weeks of inoculation in Czapek Dox Broth medium. (A) Variations in biomass. (B) Variations in medium pH. Bars represent standard errors of the mean, and a,b,c,bcdenotes mean treatments that are significantly different according to Tukey’s HSD post-hoc test at P < 0.05. (C) Micrograph of conidia harvested at 20°C with leaf and scale bar is 20 μm. (D) Culture flask with potato leaf at 20°C. (E) Culture flask with potato leaf at 38°C. (F) Culture flask without potato leaf at 20°C. (G) From left to right, supernatant of cultures medium obtain at 38°C with leaf, 28°C with leaf, 20°C with leaf and 20°C without leaf.