Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. (A) Gene expression analysis for Cochliobolus lunatus effector candidates (ClEfc) and virulence-related candidate effectors. (B) C. lunatus total RNA following DNase 1 treatment separated on a 2% denaturing formaldehydeagarose gel electrophoresis. Lane 1 (from left) represents 1 kb DNA ladder (Promega®, USA). Lane 2, 3 and 4 are total RNA extracted from mycelium grown at 20°C without potato leaf, 20°C with potato leaf and 38°C with potato leaf at the same time point, respectively. Expression of C. lunatus GAPDH was used as internal control.