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Table 1 Oligonucleotide primers for amplification of X. laevis ADHs

From: The Xenopusalcohol dehydrogenase gene family: characterization and comparative analysis incorporating amphibian and reptilian genomes

Name Nucleotide sequence1 Amino acid residues2
Degenerate primers (based on Rana perezi ADH8)
Degenerate forward outer 5′-ATGTGCACTGCGGGIAARGWIATHA-3′ 1-8
Degenerate reverse outer 5′-TAGTCTTTTGGRTTIADRCAYTC-3′ 237-244
Degenerate forward inner 5′-ATTACATGTAAGGCIGCIGTIGC-3′ 7-14
Degenerate reverse inner 5′-GGCTTTTGGRAAYTTRTCYTTRT-3′ 223-230
Specific forward primers (used in combination with 3′-end adaptors)
ADH1B outer 5′-GTATAGTGGAAAGTGTGGGAGAG-3′ 72-79
ADH1B inner 5′-CATACATTGGACTCTTGTTGGAC-3′ 119-126
ADH3 outer 5′-CTGAATACACTGTTGTAGC-A-3′ 148-154
ADH3 inner 5′-CAACTGGTTATGGAGCTGTG-3′ 178-184
ADH8B outer 5′-AGCACTTTTACAGAATACAG-3′ 144-150
ADH8B inner 5′-AGATTCCTCCAGGATCTACG-3′ 186-192
ADH9 outer 5′-GCTAAAGTACAGCAAGGTAG-3′ 189-194
ADH9 inner 5′-GAATCATTGGAGTAGACATT-3′ 219-225
Specific reverse primers for ADH8B (used in combination with the above specific forward primers)
ADH8B reverse outer 5′-TCAACAGGATGTCAGGCTGCAAATG-3′ Nucleotides 61–37 of non-coding 3′-end
ADH8B reverse inner 5′-AATGACCGTAGTGGACTTCACACGA-3′ Nucleotides 40–16 of non-coding 3′-end
  1. 1Degenerate nucleotides are R (A or G), W (A or T), H (A or C or T) and I (inosine, able to base pair with any natural nucleotide). 2Numbering refers to amino acid residues unless otherwise indicated.
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BMC Genomics

ISSN: 1471-2164

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