Degenerate primers (based on Rana perezi ADH8)
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Degenerate forward outer
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5′-ATGTGCACTGCGGGIAARGWIATHA-3′
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1-8
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Degenerate reverse outer
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5′-TAGTCTTTTGGRTTIADRCAYTC-3′
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237-244
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Degenerate forward inner
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5′-ATTACATGTAAGGCIGCIGTIGC-3′
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7-14
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Degenerate reverse inner
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5′-GGCTTTTGGRAAYTTRTCYTTRT-3′
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223-230
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Specific forward primers (used in combination with 3′-end adaptors)
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ADH1B outer
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5′-GTATAGTGGAAAGTGTGGGAGAG-3′
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72-79
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ADH1B inner
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5′-CATACATTGGACTCTTGTTGGAC-3′
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119-126
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ADH3 outer
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5′-CTGAATACACTGTTGTAGC-A-3′
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148-154
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ADH3 inner
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5′-CAACTGGTTATGGAGCTGTG-3′
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178-184
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ADH8B outer
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5′-AGCACTTTTACAGAATACAG-3′
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144-150
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ADH8B inner
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5′-AGATTCCTCCAGGATCTACG-3′
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186-192
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ADH9 outer
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5′-GCTAAAGTACAGCAAGGTAG-3′
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189-194
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ADH9 inner
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5′-GAATCATTGGAGTAGACATT-3′
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219-225
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Specific reverse primers for ADH8B (used in combination with the above specific forward primers)
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ADH8B reverse outer
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5′-TCAACAGGATGTCAGGCTGCAAATG-3′
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Nucleotides 61–37 of non-coding 3′-end
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ADH8B reverse inner
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5′-AATGACCGTAGTGGACTTCACACGA-3′
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Nucleotides 40–16 of non-coding 3′-end
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