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Table 6 Substrate and coenzyme-interacting residues in amphibian, reptilian, avian and mammalian ADH2

From: The Xenopusalcohol dehydrogenase gene family: characterization and comparative analysis incorporating amphibian and reptilian genomes

Enzyme Substrate-binding Coenzyme-binding
Inner Middle Outer      
48 93 140 141 57 Insertion after 115 294 318 110 306 309 47 48 51 269 271
 Mouse ADH2 T F F M K N F K Y A F L V I P T N A T
 Rat ADH2 T F F M K N F K Y A F L V I P T N A T
 Rabbit ADH2A S Y F F F K G K N V N F I I R S H A G
 Rabbit ADH2B S Y F L F E H K N V S F V I R S Y A G
 Human ADH2 T Y F F F N L K S V F L E I H T S/T* A G
 Marmoset ADH2 T Y F L F N L K N V F F E I H T T A G
 Bovine ADH2 S H F M F H F K N V S F M L H S H A G
 Ostrich ADH2 T Y F M F K I K T V F L M I R T H I N
 Turtle ADH2 S Y F M F K I K T V F L M I R S H I N
 X. tropicalis ADH2 T Y F M F K I K T V F L F L R T H I I
 Human ADH1B1 T F F L L - - - - V V Y M L R T H I R
  1. Human ADH1B1 is included for comparison with all available ADH2 enzymes. The four-residue insertion of class II enzymes is also shown. Residues of the coenzyme-binding site (in bold) are common to human ADH1B1. Residues at positions 223-224-225 are D-I-N in all cases. *Human ADH2 is polymorphic at position 51 and shows Ser or Thr [65].