|
Enzyme
|
Substrate-binding
|
Coenzyme-binding
|
|---|
|
Inner
|
Middle
|
Outer
| | | | | |
|---|
|
48
|
93
|
140
|
141
|
57
|
Insertion after 115
|
294
|
318
|
110
|
306
|
309
|
47
|
48
|
51
|
269
|
271
|
|---|
|
Mouse ADH2
|
T
|
F
|
F
|
M
|
K
|
N
|
F
|
K
|
Y
|
A
|
F
|
L
|
V
|
I
|
P
|
T
|
N
|
A
|
T
|
|
Rat ADH2
|
T
|
F
|
F
|
M
|
K
|
N
|
F
|
K
|
Y
|
A
|
F
|
L
|
V
|
I
|
P
|
T
|
N
|
A
|
T
|
|
Rabbit ADH2A
|
S
|
Y
|
F
|
F
|
F
|
K
|
G
|
K
|
N
|
V
|
N
|
F
|
I
|
I
|
R
|
S
|
H
|
A
|
G
|
|
Rabbit ADH2B
|
S
|
Y
|
F
|
L
|
F
|
E
|
H
|
K
|
N
|
V
|
S
|
F
|
V
|
I
|
R
|
S
|
Y
|
A
|
G
|
|
Human ADH2
|
T
|
Y
|
F
|
F
|
F
|
N
|
L
|
K
|
S
|
V
|
F
|
L
|
E
|
I
|
H
|
T
|
S/T*
|
A
|
G
|
|
Marmoset ADH2
|
T
|
Y
|
F
|
L
|
F
|
N
|
L
|
K
|
N
|
V
|
F
|
F
|
E
|
I
|
H
|
T
|
T
|
A
|
G
|
|
Bovine ADH2
|
S
|
H
|
F
|
M
|
F
|
H
|
F
|
K
|
N
|
V
|
S
|
F
|
M
|
L
|
H
|
S
|
H
|
A
|
G
|
|
Ostrich ADH2
|
T
|
Y
|
F
|
M
|
F
|
K
|
I
|
K
|
T
|
V
|
F
|
L
|
M
|
I
|
R
|
T
|
H
|
I
|
N
|
|
Turtle ADH2
|
S
|
Y
|
F
|
M
|
F
|
K
|
I
|
K
|
T
|
V
|
F
|
L
|
M
|
I
|
R
|
S
|
H
|
I
|
N
|
|
X. tropicalis ADH2
|
T
|
Y
|
F
|
M
|
F
|
K
|
I
|
K
|
T
|
V
|
F
|
L
|
F
|
L
|
R
|
T
|
H
|
I
|
I
|
|
Human ADH1B1
|
T
|
F
|
F
|
L
|
L
|
-
|
-
|
-
|
-
|
V
|
V
|
Y
|
M
|
L
|
R
|
T
|
H
|
I
|
R
|
- Human ADH1B1 is included for comparison with all available ADH2 enzymes. The four-residue insertion of class II enzymes is also shown. Residues of the coenzyme-binding site (in bold) are common to human ADH1B1. Residues at positions 223-224-225 are D-I-N in all cases. *Human ADH2 is polymorphic at position 51 and shows Ser or Thr [65].
