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Table 6 Substrate and coenzyme-interacting residues in amphibian, reptilian, avian and mammalian ADH2

From: The Xenopusalcohol dehydrogenase gene family: characterization and comparative analysis incorporating amphibian and reptilian genomes

Enzyme

Substrate-binding

Coenzyme-binding

Inner

Middle

Outer

     

48

93

140

141

57

Insertion after 115

294

318

110

306

309

47

48

51

269

271

 Mouse ADH2

T

F

F

M

K

N

F

K

Y

A

F

L

V

I

P

T

N

A

T

 Rat ADH2

T

F

F

M

K

N

F

K

Y

A

F

L

V

I

P

T

N

A

T

 Rabbit ADH2A

S

Y

F

F

F

K

G

K

N

V

N

F

I

I

R

S

H

A

G

 Rabbit ADH2B

S

Y

F

L

F

E

H

K

N

V

S

F

V

I

R

S

Y

A

G

 Human ADH2

T

Y

F

F

F

N

L

K

S

V

F

L

E

I

H

T

S/T*

A

G

 Marmoset ADH2

T

Y

F

L

F

N

L

K

N

V

F

F

E

I

H

T

T

A

G

 Bovine ADH2

S

H

F

M

F

H

F

K

N

V

S

F

M

L

H

S

H

A

G

 Ostrich ADH2

T

Y

F

M

F

K

I

K

T

V

F

L

M

I

R

T

H

I

N

 Turtle ADH2

S

Y

F

M

F

K

I

K

T

V

F

L

M

I

R

S

H

I

N

 X. tropicalis ADH2

T

Y

F

M

F

K

I

K

T

V

F

L

F

L

R

T

H

I

I

 Human ADH1B1

T

F

F

L

L

-

-

-

-

V

V

Y

M

L

R

T

H

I

R

  1. Human ADH1B1 is included for comparison with all available ADH2 enzymes. The four-residue insertion of class II enzymes is also shown. Residues of the coenzyme-binding site (in bold) are common to human ADH1B1. Residues at positions 223-224-225 are D-I-N in all cases. *Human ADH2 is polymorphic at position 51 and shows Ser or Thr [65].
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BMC Genomics

ISSN: 1471-2164

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