Co-segregation of the FaFAD1 , PCR-based marker with the γ–decalactone phenotype. The FaFAD1-based PCR product is denoted by the single arrow and migrates at 500 bp by design. The dashed arrow denotes a positive PCR control (BFACT045) that is located in proximity of FaFAD1. Plus and minus signs represent the γ–decalactone phenotype for each genotype as determined by GC/MS. (A) The parental lines ‘Elyana’ x ‘Mara des Bois’ are shown with a subset of their progeny. (B) A series of wild octoploid materials representing one F. chiloensis and seven F. virginiana accessions (number = GRIN PI accession). (C) The correlation of the PCR product and γ–decalactone phenotype in cultivars unrelated to populations described in this work.