Table 1 Primers used to perform qPCR of selected candidates
All conditions | |||
|---|---|---|---|
miRNA | Sequence | Forward primer | Reverse primer |
mir-2-5p | UAUCACAGCCAGCUUUGAUGUGC | TCACAGCCAGCTTTGATG | GTCCAGTTTTTTTTTTTTTTTGCAC |
mir-46-3p | UGUCAUGGAGUCGCUCUCUUCA | TGTCATGGAGTCGCTCTC | GGTCCAGTTTTTTTTTTTTTTTGAAGA |
mir-47-3p | UGUCAUGGAGGCGCUCUCUUCA | CATGGAGGCGCTCTC | GGTCCAGTTTTTTTTTTTTTTTGAAGA |
UV | |||
mir-357-3p | AAAUGCCAGUCGUUGCAGGAGU | TGCCAGTCGTTGCAG | GGTCCAGTTTTTTTTTTTTTTTACTC |
miR-57-5p | UACCCUGUAGAUCGAGCUGUGUGU | AGTACCCGTATAAGTTTCTGCT | GGTCCAGTTTTTTTTTTTTTTTCTC |
miR-55-3p | UACCCGUAUAAGUUUCUGCUGAG | GCAGTATGACACTGAAGCGT | GTCCAGTTTTTTTTTTTTTTTCGGT |
Heat-stress | |||
miRNA | Sequence | Forward primer | Reverse primer |
miR-64-5p | UAUGACACUGAAGCGUUACCGAA | GCAGTATGACACTGAAGCGT | GTCCAGTTTTTTTTTTTTTTTCGGT |
miR-86-5p | UAAGUGAAUGCUUUGCCACAGUC | AGTAAGTGAATGCTTTGCCA | GTCCAGTTTTTTTTTTTTTTTGAC |
miR-794-5p | UGAGGUAAUCAUCGUUGUCACU | CAGTGAGGTAATCATCGTTGT | GTCCAGTTTTTTTTTTTTTTTAGTGA |
Hypoxia N2 + hif-1 | |||
miRNA | Sequence | Forward primer | Reverse primer |
miR-230-3p | GUAUUAGUUGUGCGACCAGGAGA | GGTATTAGTTGTGCGACCAG | GGTCCAGTTTTTTTTTTTTTTTCTC |
miR-357-3p | AAAUGCCAGUCGUUGCAGGAGU | TGCCAGTCGTTGCAG | GGTCCAGTTTTTTTTTTTTTTTACTC |
miR-58-3p | UGAGAUCGUUCAGUACGGCAAU | CAGTGAGATCGTTCAGTACG | GGTCCAGTTTTTTTTTTTTTTTATTGC |
Hypoxia N2 | |||
miRNA | Sequence | Forward primer | Reverse primer |
miR-52-5p | CACCCGUACAUAUGUUUCCGUGCU | ACCCGTACATATGTTTCCGT | GGTCCAGTTTTTTTTTTTTTTTAGCA |
miR-254 | UGCAAAUCUUUCGCGACUGUAGG | GCAAATCTTTCGCGACTGT | GGTCCAGTTTTTTTTTTTTTTTCCT |
miR-241-3p | AUUGUCUCUCAGCUGCUUCAUC | AGATTGTCTCTCAGCTGCT | GTCCAGTTTTTTTTTTTTTTTGATGAAG |