Skip to main content

Table 3 Summary of the horseradish peroxidase isoenzymes and associated data produced during this study

From: Peroxidase gene discovery from the horseradish transcriptome

Contig number Name (* novel) Length nt GC content% Accession # CAI calculated Intron # Signal sequence length Disulfide bridges (EDBCP prediction) pI mature protein
- C1A 1062 43.69 HE963800 0.812 3 30 [11–91] [44-49] [97–301] [177–209] 5.59
15901 C1B 1056 43.94 HE963801 0.808 3 28 [11–91] [44-49] [97–301] [177–209] 5.84
25148 C1C* 1059 45.14 HE963802 0.809 3 29 [11–91] [44-49] [97–301] [177–209] 6.49
25148_2 C1D* 1059 45.04 HE963803 0.810 3 29 [11–91] [44-49] [97–301] [177–209] 7.04
04627 C2 1044 42.91 HE963804 0.800 3 24 [11–91] [44-49] [97–301] [177–209] 8.56
- C3 1050 46.76 HE963805 0.781 3 29 [11–91] [44-49] [97–300] [177–209] 7.71
Manual assembly A2A* 1011 46.79 HE963806 0.761 3 31 [11–91] [44-49] [97–299] [176–208] 4.93
Manual assembly A2B* 1011 46.69 HE963807 0.761 3 31 [11–91] [44-49] [97–299] [176–208] 4.93
04382 E5 1044 46.07 HE963808 0.771 3 27 [11–91] [44-49] [97–300] [177–209] 8.84
01805 1805* 1065 44.41 HE963809 0.797 3 31 [11–91] [44-49] [97–301] [177–209] 5.97
22684 22684.1* 1050 46.76 HE963810 0.770 3 29 [11–91] [44-49] [97–300] [177–209] 6.98
22684_2 22684.2* 1050 46.67 HE963811 0.772 3 29 [11–91] [44-49] [97–300] [177–209] 6.37
01350 1350* 975 50.97 HE963812 0.707 3 28 [11–91] [44-49] [97–292] [176–201] 8.67
02021 2021* 996 46.08 HE963813 0.788 3 29 [11–89] [44-49] [95–297] 9.46
23190 23190.1* 1080 49.26 HE963817 0.724 2 31 42 [11–92] [44-49] [98–293] [178–205] or [22–103] [55-60] [109–304] [189–216] 8.40 6.58
23190_2 23190.2* 1080 49.17 HE963817 0.722 2 31 42 [11–92] [44-49] [98–293] [178–205] or [22–103] [55-60] [109–304] [189–216] 8.60 7.09
04663 4663* 1077 47.82 HE963814 0.748 3 31 [11–91] [44-49] [97–299] [176–208] 4.48
06351 6351* 945 43.39 HE963816 0.786 3 18 [17–96] [50-55] [102–292] [180–206] 6.37
03523 3523* 960 44.58 HE963820 0.761 0 22 [11–92] [44-49] [98–293] [177–203] 8.99
05508 5508.1* 966 49.28 HE963815 0.735 2 24 30 [11-87] [44-49] [93–287] [171–198] or [17–93] [50-55] [99–293] [177–204] 8.49 8.47
05508_2 5508.2* 966 49.38 HE963815 0.735 2 24 30 [11-87] [44-49] [93–287] [171–198] or [17–93] [50-55] [99–293] [177–204] 8.49 8.47
22489_1 22489.1* 978 51.02 HE963818 0.726 2 23 34 [22–98] [55-60] [104–298] [182–209] or [11-87] [44-49] [93–287] [171–198] 8.93 8.51
22489_2 22489.2* 978 50.82 HE963819 0.727 2 23 34 [22–98] [55-60] [104–298] [182–209] or [11-87] [44-49] [93–287] [171–198] 8.93 8.51
06117 6117* 1008 47.02 HE963821 0.802 3 22 32 [11–91] [44-49] [97–298] [176–208] or [21–101] [54-59] [107–308] [186–218] 5.52 6.16
17517_1 17517.1* 972 48.46 HE963822 0.737 2 23 24 [12–88] [45-50] [94–296] [171–203] or [11-87] [44-49] [93–295] [170–202] 9.49 9.39
17517_2 17517.2* 972 48.56 HE963823 0.739 2 23 24 [12–88] [45-50] [94–296] [171–203] or [11-87] [44-49] [93–295] [170–202] 9.52 9.41
08562_1 08562.1* 996 47.09 HE963824 0.779 3 22 28 [11–91] [44-49] [97–298] [176–208] or [17–97] [50-55] [103–304] [182–214] 9.01 9.03
08562_4 08562.2* 996 47.79 HE963825 0.788 3 22 28 [11–91] [44-49] [97–298] [176–208] or [17–97] [50-55] [103–304] [182–214] 9.00 9.02
  1. The nucleotide sequences of 28 isoenzymes were submitted to EMBL. “*” indicates novel. “CAI” = codon adaptation index. If signal sequence predictions gave more than one alternative result, both signal sequence lengths are shown, separated by “/”. Disulfide bridges were predicted using both alternatives of the mature protein (EDBCP = Ensemble-based Disulfide Bonding Connectivity Pattern prediction server).