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Table 3 Predicted mRNA binding targets of sRNA-1 and sRNA-2 on pXO1

From: Transcriptome analysis identifies Bacillus anthracis genes that respond to CO2through an AtxA-dependent mechanism

Small RNA

Closest ORF (Locus Tag)

Protein name or ID

Region of predicted mRNA

Region of redicted sRNA

Energy of interaction (kJ/mol)

Z-score

sRNA-1

pXO1_0199

relA-like

184-196

125-137

−13.98

−2.13

pXO1_0146

AtxA

(-138)-(-129)

75-84

−13.53

−2.01

pXO1_0113

YP_016444.2

239-259

62-82

−12.79

−1.82

pXO1_0080

S-layer domain protein

637-653

116-137

−12.47

−1.73

pXO1_0182

Integrase/recombinase

20-29

75-84

−12.05

−1.62

sRNA-2

pXO1_0066

YP_016397.2

1079-1103

61-86

−14.47

−3.21

pXO1_0067

RepX (FtsZ-like)

268-278

68-78

−13.36

−2.72

pXO1_0010

YP_016341.2

178-199

61-78

−13.32

−2.7

pXO1_0130

UDP-glucose 6-dehydrogenase

1006-1023

69-86

−12.53

−2.35

pXO1_0113

YP_016444.2

107-132

77-104

−12.52

−2.35

  1. RNAPredator predictions of the five mRNA targets having the lowest Z-scores for each sRNA are listed. Z-scores quantify the deviation of the binding energy for that interaction from the set of all possible sRNA-mRNA binding interactions.