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Table 5 Mismatch identification

From: High-throughput transcriptome sequencing and preliminary functional analysis in four Neotropical tree species

 

Carapa guianensis

Eperua falcata

Symphonia globulifera

Virola surinamensis

Total length with depth ≥ 8X after assembly cleaning (bases)

956876

603897

499694

862357

Before post-filtering based on binomial test

N mismatches

10615

7084

5447

10897

N variant-containing contigs

1716 (7.88%)

1299 (5.55%)

987 (5.77%)

1752 (8.32%)

mismatch density (/100 bp)

1.11

1.17

1.09

1.26

N mismatches with 2 variants

10420 (98.16%)

6968 (98.36%)

5362 (98.44%)

10757 (98.72%)

N transitions

2655

1875

1090

2182

N transversions

1699

1155

779

1474

Ti/Tv

1.56

1.62

1.40

1.48

N indel

6066

3938

3493

7101

N mismatches > 2 variants

195 (1.84%)

116 (1.64%)

85 (1.56%)

140 (1.28%)

After post-filtering based on binomial test

N mismatches

8646

5713

4434

9076

N variant-containing contigs

1706 (7.83%)

1283 (5.5%)

979 (5.72%)

1746 (8.29%)

mismatch density (/100 bp)

0.90

0.95

0.89

1.05

N mismatches with 2 variants

8534 (95.70%)

5649 (98.89%)

4388 (98.96%)

8981 (98.95%)

N transitions

2380

1657

989

1989

N transversions

1488

1000

681

1310

Ti/Tv

1.60

1.66

1.45

1.52

N indel

4666

2992

2718

5682

N mismatches > 2 variants

112 (1.13%)

64 (1.12%)

46 (1.04%)

95 (1.05%)