Gene expression profile analysis of Manila clam (Ruditapes philippinarum) hemocytes after a Vibrio alginolyticus challenge using an immune-enriched oligo-microarray

Table 1 Description of the microarray design process

	n° sequences
Sequence origin
Sanger (Milan et al. [20])	5,758
454 tissues (Milan et al. [20])	457,717
454 hemocytes (Moreira et al. [21])	975,190
subTOTAL	1,438,665
NCBI	2,050
TOTAL	1,440,715
Assembly
Singletons	169,223
Singletons phred Q > 20	16,495
Isotigs	26,708
Isogroups	15,175
Contigs	156
Annotation
Singletons phred Q > 20 and NCBI	5,914
Contigs and longest isotig of each isogroup	6,242
Frame +	7,827
Frame -	2,761
Ambiguous frame	1,568
TOTAL R. philippinarum successfully designed probes	13,671

ISSN: 1471-2164