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Table 2 Melting temperatures (Tm) determined for French-specific canSNP by HRM

From: High-throughput sequencing of Bacillus anthracis in France: investigating genome diversity and population structure using whole-genome SNP discovery

canSNP

Region

Allele

Tm values (°C)

Nb strains

BA15

Alps

G

75.16 ± 0.05

38

Others

A

74.61 ± 0.07

100

BA16

Pyr

G

79.93 ± 0.04

9

Others

A

79.39 ± 0.08

129

BA17

MCSL

A

77.27 ± 0.08

26

Others

G

77.87 ± 0.13

112

BA18

SL

C

79.83 ± 0.10

6

Others

T

79.13 ± 0.10

132

BA19

France

G

75.84 ± 0.07

73

Others

A

75.22 ± 0.07

65

BA20

Doubs

C

80.36 ± 0.05

21

Others

A

79.83 ± 0.06

117

BA21

CNE

C

77.90 ± 0.05

15

Others

A

77.08 ± 0.08

123

BA22

NE

C

77.50 ± 0.08

10

Others

T

76.93 ± 0.08

128

  1. Tm values are means ± standard deviation calculated from 138 strains. MCSL: Massif Central and Saône-et-Loire; SL: Saône-et-Loire; Pyr: Pyrenees; CNE: Central North-Eastern ; NE: North-Eastern.