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Figure 6 | BMC Genomics

Figure 6

From: Genome-wide transcriptome study in wheat identified candidate genes related to processing quality, majority of them showing interaction (quality x development) and having temporal and spatial distributions

Figure 6

Validation of differential expression (fold change) of two randomly chosen candidate genes (pre-α/β-gliadin and γ-Gliadin) at three seed development stages using qRT-PCR. The validation was done between a good (C306) and a poor (Sonalika) chapatti quality varieties in 7, 14, and 28 days after anthesis (DAA) of seed developmental stage through quantitative real time PCR (qRT-PCR). Y-axis represents fold change in differential expression of genes in the wheat variety, C306 in comparison to the wheat variety, Sonalika. X-axis represents three seed development stages i.e. 7, 14, and 28 DAA. The expression data were normalized to that of a control gene, ADP ribosylation factor, ARF. qRT-PCR data analysis was done following Livak and Schmitteng (2001) [46].

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BMC Genomics

ISSN: 1471-2164

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