Figure 2

Global changes in gene expression to novobiocin treatment. Gene expression differences between novobiocin treated (0.5 mg/L) and untreated HG001 determined by microarray analysis and illustrated by Voronoi Treemap (sub-function categories). Strain HG001 was grown to exponential phase (OD₆₀₀ = 0.6) and treated with and without novobiocin (0.5 mg/L) for one hour. Triplicate RNA samples were processed and further analyzed with Affymetrix gene chip. All functionally annotated genes of the S. aureus Affymetrix array are displayed and allocated to TheSeed functional categories. Clustering in groups or categories means functional or systematic relationship. If a gene or a group of genes fulfills multiple functions, it will be allocated to more than one functional group and by this reason appear at multiple locations within the treemap. Voronoi tree map is shown by a colour ramp from dark blue (at least 0.5 (log₂fold) repressed in novobioin treated bacteria) via light grey (unchanged) to dark orange (at least 0.5 (log₂fold) induced in novobiocin treated cells).