Figure 5

Keratin induction and reorganization of intermediate- and micro-filaments under hypoxia. (A) Western blot of Keratin-16 expression in normoxic (N-MSC) and hypoxic (H-MSC) human MSCs. (B) Keratin immunostaining and (C) F-actin immunostaining of MSCs cultured for 24 h under normoxia (N-MSC) or hypoxia (H-MSC). Western blots and immunostaining are described in Methods. All results are representative of 3 separate experiments; *p < 0.05, n = 3.